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文献和实验Besides stimulating the mitogen-activated protein kinase, phospholipase Cγ, and phosphatidylinositol 3-kinase cascades, in certain tissues and cells such as the heart, partotid gland, and luteal cells, activation of the epidermal growth
Measurement of Phospholipase C Activity in Brain Membranes
(1,4,5)P 3 ] and 1,2-diacyl-glycerol ( 1 – 3 ). Coupling between the receptor and PLC is often regulated by a guanine nucleotide binding regulatory protein from the G q family ( 4 ), similar to those involved in the regulation of adenylyl cyclase
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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