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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
保存:Freezer
- 保质期:
咨询甄准
- 英文名:
1000ug/mL Paraldehyde in MeOH P+T
- 库存:
咨询甄准
- 供应商:
上海甄准
- 规格:
1 mL
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文献和实验In Situ Hybridization Protocols
5 minutes at room temperature 50% MeOH : 50% PTw 25% MeOH : 75% PTw 100% PTw Wash twice in PTw for 5 minutes each. Treat embryos with 10ug/ml proteinase K
Anderson Lab In Situ Hybridization Protocols
) -------------------------------------------------- = t (0.11) (Starting length, kb) (Desired length, kb) Precipitate hydrolysed probe again as in (8) above. Resuspend the probe in hybridisation buffer to a final concentration of 10ug/ml
Dissociated Retina Immunohistochemistry
Solutions 100X trypsin 10 mg/ml in 1X PBS Sigma #T2271 100X soybean trypsin inhibitor 10 mg/ml in 1X PBS Sigma #T6522 1000X DNaseI 2mg/ml in HBSS Sigma #D4513 Explant Culture
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