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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
保存:Freezer
- 保质期:
咨询甄准
- 英文名:
1000 ug/mL Triphenyl phosphorothionate in Methanol
- 库存:
咨询甄准
- 供应商:
上海甄准
- 规格:
1 mL
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文献和实验1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary
Single and Double FISH protocols for Drosophila
1. Chlorine bleach; diluted 1:1 with water. 2. 40% formaldehyde solution (prepared fresh from paraformaldehyde as described below). 3. 10X PBS solution. 4. Heptane. 5. Methanol. 6. 20 ml Disposable Glass Scintillation Vials (Fisher).
Antibody Staining of Dissected Gonads
). DAPI -- 100 ug/ml stock solution. Dilute 1:1000 in PBS Levamisole (Sigma) stock: 100 mM in dH20 (store @ -200C). Methanol: 100% stock kept at -20 0C
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