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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
保存:室温
- 保质期:
咨询甄准
- 英文名:
Base/Neutral Fraction Calibration Check Compounds
- 库存:
咨询甄准
- 供应商:
上海甄准
- 规格:
1 mL
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文献和实验Lipid analysis-Week 3: GAS LIQUID CHROMATOGRAPHY
of chemicals, and it is perfect for the analysis of fatty acid components. Like in any other chromatographic technique, separation of compounds depends on their partition between a stationary and a mobile phase. In gas chromatography, the mobile phase
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
buffer (fraction S2). Dialyse S1 and S2 overnight at 4°C against 2 litres of Lysis buffer (see note 7); Centrifuge dialysed chromatin at 1800 x g for 10 minutes at 4°C. Remove supernatants and place on ice (fractions S1 and S2); Resuspend
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