- 询价
- SPEX CertiPrep
- 国外
- ZSC-CAN-CAN-4
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
保存：室温
- 保质期：
咨询甄准
- 英文名：
Canadian Pesticide Mix 4 Containing 19 Compounds
- 库存：
咨询甄准
- 供应商：
上海甄准
- 规格：
1 mL
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文献和实验at room temperature for 10 min. 11. Transfer the upper, aqueous phase to a new 50 ml tube. 12. Add two-thirds volume of cold 100% Isopropanol to the tube and mix gently to precipitate the nucleic acids (see Hint #2). 13. Centrifuge at 5,000 X g at room
to the tube, gently swirling. 10. Centrifuge at 16,000 X g at room temperature for 10 min. 11. Transfer the upper, aqueous phase to a new 50 ml tube. 12. Add two-thirds volume of cold 100% Isopropanol to the tube and mix gently to precipitate
, trypsinize and split 1:5 into SILEC expansion medium. For S2 (suspension) cells, when cells have reached ~1 × 107 cells per ml, split into a new flask containing SILEC expansion medium at a concentration of 2 × 106 cells. (3) Repeat Step
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