- 询价
- SPEX CertiPrep
- 国外
- ZSC-USP-PARENT2B-1
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
保存：室温
- 保质期：
咨询甄准
- 英文名：
USP 232 Revision 40 Parenteral 2B Mix 1
- 库存：
咨询甄准
- 供应商：
上海甄准
- 规格：
125 mL
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文献和实验DIRECT AND SHORT-TERM PROCEDURE FOR HARVESTING BONE MARROW CHROMOSOMES
into tube A, add 65 mcl colcemid working solution to the tube. Pipet 10 ml of complete media into tube B, add 45 mcl EtBr and 20 mcl 0.5X colcemid working solution. Add 7 to 9 drops of bone marrow aspirate to each tube; seal tubes tightly, invert to mix
, Cas9 mediates cleavage of target DNA upstream of PAM to create a DSB within the protospacer. ( b ) Design of chimeric sgRNA for genome cleavage. The SpCas9 nuclease ( yellow ) binds to genomic DNAdirected by the sgRNA consisting of a 20-nt guide
Buffer, # N808-0189, and Ampli-Taq DNA polymerase, # N808-4015 store frozen, -20°C (Perkin Elmer, Norwalk, CT) Carbenicillin (Gibco-BRL, Rockville, MD) Ethanol (200 Proof USP Ethyl Alcohol) 1M Tris-HCl (pH 8) 0.5M NaEDTA (pH 8) T Low E Buffer
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