Procollagen Type III rabbit po

gene_symbol：null
predicted_size：null
Uniprot ID
description：Procollagen Type III rabbit polyclonal antibody, Purified
clonality：Polyclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.
immunogen：Purified Human Procollagen type III C-terminal and N-terminal PIIICP separated.
recommended_dilution：RIA: > 1/200. ELISA: 1/100-1/200 (OD ≥ 500).  Immunofluorescence (indirect, 1/40 on Frozen tissues). Immunohistochemistry on Frozen and Paraffin Embedded Tissue Sections: 1/500. Pretreatment : After removing Paraffin pre-treat with 0.2% Hyaluronidase (approx. 300 U/mg) in TBS, 15 min at 37°C, then block unspecific binding with blocking serum or 3% BSA in TBS and perform blocking of endogen Peroxidase with 1% H2O2 in TBS, if necessary. Incubation Time : 60 min at room temperature or overnight at 2-8°C. Positive Control : Human or Bovine Skin and Liver.
predicted_size：null
buffer：PBS without BSA or preservatives; State: Purified; State: Lyophilized purified Ig fraction
host_species：Rabbit
applications：ELISA, IF, IHC, R
reactivities：Bovine, Human, Porcine