TNFRSF1B mouse monoclonal anti

gene_symbol：TNFRSF1B
predicted_size：null
Uniprot ID：P2333
description：TNFRSF1B mouse monoclonal antibody, clone utr1, Azide Free
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain.
TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.
immunogen：Partially purified preparations of TNF binding proteins from U937 cells.
recommended_dilution：Immunohistology: 2-5 µg/ml on Cryostat Sections (2% Periodate-lysine-paraformaldehyde or acetone for fixation). Flow Cytometry: 10 µg/ml. Immunoprecipitation. Western blots: 30 µg/ml on non-reduced SDS PAGE. The epitope is sensitive to reduction, may need enrichment by Immunoprecipitation. Functional Assay: This antibody utr1 is useful for studying biological effects of TNF-Receptor p75 in vitro where it inhibits the binding of radiolabelled TNF to Human cells expressing the p75 TNF receptor. In order to completely block TNF binding to the cell surface, 10 µg/ml of utr1 are required. utr1 itself may have an agonistic effect in assays measuring cytotoxicity, fibroblast growth or IL-6 secretion.  Recommended Positive Control: Human Tonsil.
predicted_size：null
buffer：PBS, pH 7.2; State: Azide Free; State: Liquid purified IgG fraction from Culture Supernatant; Stabilizer: None; Preservative: None
host_species：Mouse
applications：FC, FN, IHC, IP, WB
reactivities：Human