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10 mg
predicted_size:null
Uniprot ID:P584
description:uidA rabbit polyclonal antibody, Azide Free
clonality:Polyclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Primary Antibodies
background:Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.
immunogen:Beta-Glucuronidase is isolated and purified from Escherichia coli. Freund's complete adjuvant is used in the first step of the immunization procedure.
recommended_dilution:Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice. Recommended Working Dilutions : vary widely, but may be up to 1/10000.
predicted_size:null
buffer:PBS, pH 7.2 without preservatives and foreign proteins; State: Azide Free; State: Lyophilized Hyperimmune IgG fraction
host_species:Rabbit
applications:ELISA, ID, IF, IP, R, WB
reactivities:Escherichia coli
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文献和实验Polyclonal Antibody Production
Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Generation and Selection of Rabbit Antibody Libraries by Phage Display
The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs
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