PF4 mouse monoclonal antibody,

gene_symbol：PF4
predicted_size：null
Uniprot ID：P2776
description：PF4 mouse monoclonal antibody, clone KKO, Purified
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity.

Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies.
These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FCGIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation.
immunogen：Human Factor IV purified from the supernatant of Thrombin activated platelets.
recommended_dilution：ELISA: Use at 0.08 μg/ml following coating plate with PF4/ heparin complex. This can be done by incubating PF4 with an excess of heparin (100-200 U/ml), while coating the plate. Western blot.
predicted_size：null
buffer：PBS; State: Purified; State: Liquid purified IgG fraction; Preservative: 0.02% Sodium Azide
host_species：Mouse
applications：ELISA, WB
reactivities：Human