TNF alpha (TNF) mouse monoclon

gene_symbol：TNF
predicted_size：null
Uniprot ID：P1375
description：TNF alpha (TNF) mouse monoclonal antibody, clone B1E4, Aff - Purified
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
immunogen：Purified recombinant Human TNF alpha.
recommended_dilution：ELISA: The detection limit of ELISA can reach 2.4 pg/ml when clone B1E4 is used as Tracer and clone CH8820 Cat.-No DM1049 is used as Coating antibody. Western Blotting: The antibody reacts with recombinant 17kDa TNF alpha.
predicted_size：null
buffer：0.01M PBS, pH 7.0 without preservatives.; State: Aff - Purified; State: Lyophilized purified IgG fraction.
host_species：Mouse
applications：ELISA, WB
reactivities：Human