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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 保质期:
1年
- 英文名:
Oligo dT(18)(10μM)
- 库存:
100
- 供应商:
臻诺生物
- 规格:
100 μl
凯基 KGDN12 Oligo dT(18)(10μM)

凯基 KGDN12 Oligo dT(18)(10μM)

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文献和实验Synthesis of Radiolabeled, Subtracted cDNA Probes Using Oligo(dT) as a Primer
2. To the chilled microfuge tube, add: 0.1 M dithiothreitol 2.5 μl placental RNase inhibitor 200 units oligo(dT)12-18 10 μl 10x reverse transcriptase buffer 25 μl 20 mM solution of dGTP, dATP, and dTTP 10 μl 125 μM dCTP 10 μl 10 mCi/ml
for large-scale purification (>25 µg) of poly(A)+ RNAextracted from mammalian cells. Typically, between 1% and 10% of the RNA applied to the oligo(dT) column is recovered as poly(A)+ RNA. Because the method can be frustratingly slow, it is not recommended
Screening Poly [dA/dT()] cDNA for Gene Identification
, we believe that the use of complementary DNA (cDNA) libraries generated by regular oligo-(dT) primers contributes significantly to this problem (10 ,11 ). The cDNAs generated by oligo-(dT) priming contain various lengths of poly(dA/dT) tail sequences
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