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|---|---|---|---|
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文献和实验Sphingosine Kinase: Assay and Product Analysis
), and inhibition of ceramide-induced activation of stress-activated protein kinase (SAPK/JNK) that leads to apoptotic responses (15 ). Additional effects of SPP include stimulation of tyrosine phosphorylation of focal adhesion kinase (FAK) and the cytoskeleton
High-Throughput Kinase Assay Based on Surface Plasmon Resonance
, and phospho-tyrosine (pTyr) residues are detected by an anti-pTyr antibody. The level of tyrosine phosphorylation is calculated from the capture level of the substrates and the binding level of the anti-pTyr antibody monitored by SPR. A wide dynamic range
Detection of RTK Pathway Activation in Drosophila Using Anti-dpERK Immunofluorescence Staining
In Drosophila , like in other metazoans, receptor tyrosine kinase (RTK) signaling pathways control diverse cellular processes such as migration, growth, fate determination, and differentiation (Shilo, Development 132:4017–4027, 2005
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