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- 详细信息
- 文献和实验
- 技术资料
- 库存：
89
- 供应商：
上海淳麦
- 检测方法：
ELISA
- 适应物种：
人
- 样本：
细胞培养上清、血清、血浆、尿液、组织样本等
- 规格：
48T/96T
产品名称：Human myosin light chain kinase,MLCK ELISA Kit
用途
该试剂盒用于体外定量检测人血清、血浆或其他相关生物液体中天然及部分Human myosin light chain kinase,MLCK ELISA Kit需自备的设备及试剂
1、450±10nm 滤光片的酶标仪(建议仪器使用前提前预热)
2、单道或多道微量加液器及吸头
3、稀释样品的EP 管
4、蒸馏水或去离子水
5、吸水纸
6、盛放洗液的容器
Human myosin light chain kinase,MLCK ELISA Kit
Human myosin light chain kinase,MLCK ELISA Kit标本的采集与保存:
Human myosin light chain kinase,MLCK ELISA Kit
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文献和实验Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
Role of Rho, Rac, and Rho-kinase in phosphorylation of myosin light chain, development of polarity, and spontaneous migration of Walker 256 carcinosarcoma cells.As previously shown, constitutive activation of the small GTPase Rho and its downstream
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