Human Ig lambda-2 chain C regi

Introduction of human 14-3-3 proteins

of tyrosine and tryptophan hydroxylases and for inhibition of Ca2+/phospholipid-dependent protein kinase C.Northern blot analysis demonstrated widespread expression of the 14-3-3 protein eta chain in cultured cell lines derived from various human tumors

Mapping, PCR DNA Sequencing

chain termination separation of the resulting molecules by polyacrylamide gel electrophoresis. The DNA fragment to be sequenced must first be cloned into a vector (plasmid or lambda). Then the cloned DNA must be copied in a test tube (in vitro

【分享】ELISA原理

等。抗原的反应性取决于抗原决定簇（antigenic determinant），或称为表位（epitope）。一个抗原分子可带有不同的决定簇。1.2　抗体1.2.1　抗体的结构抗体是能与抗原特异性结合的免疫球蛋白(immunoglobulin,Ig)。Ig分五类，即IgG、IgA、IgM、IgD和IgE。与免疫测定有关的Ig主要为IgG和IgM。Ig由两个轻链（L）和两个重链（H）的单体组成。Ig的轻链是相同的，有κ（kappa）和λ（Lambda）两种型别。五类Ig的重链结构不同，这决定了它们的抗原