Human Histone H2A type 1-A(HIS

Native Chromatin Preparation and Illumina/Solexa Library Construction

Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies

Detection of a Lipid-Lysine Adduct Family with an Amide Bond as the Linkage: Novel Markers for Lipid-Derived Protein Modifications

An amide-type adduct, hexanoyl-lysine (HEL) is generated from the reaction between n-6 fatty acid (FA)-derived lipid peroxide and lysine. Immunochemical and chemical methods can be used to detect the formation of HEL. For example, an ELISA

Isolation of Histones and Nucleosome Cores from Mammalian Cells

is often important for understanding mechanisms of regulation of transcription and other processes that occur on DNA. The basic unit of chromatin is the nucleosome core, containing two copies each of the core histones H2A, H2B, H3, and H4 to form a histone octamer