Human Disks large homolog 3(DL

siRNAs结合生物芯片的实验设计

should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example

利用人工组合转录因子对人类基因组扫描

large combinatorial libraries of artificial transcription factors comprising three or six zinc-finger domains, and selected transcription factor�DNA interactions able to upregulate several genes in human cells. Selected transcription factors

RNAi和基因沉默的历史回顾

that soaking worms in dsRNA was also able to induce silencing (14). These strategies, whereby large numbers of nematodes are exposed to dsRNA, have enabled large-scale screens to select for RNAi-defective C. elegans mutants and have led to large numbers of gene