Human Cytokine receptor-like f

Mast Cell Histamine and Cytokine Assays

Mast cells are crucial to the development of chronic allergic inflammation and are likely to play a critical role in host defense. In this chapter methodology for histamine and cytokine assays is provided. Crosslinkage of IgE receptor I (Fcε

Cytokine Bioassays

to a specific growth factor. Other aspects of biological activity of cytokines include induction of further cytokine secretion, induction of killing, antiviral activity, degranulation, cytotoxicity, chemotaxis, and promotion of colony formation. In vitro assays

Overview of Approaches to the Identification of Inhibitors of Cytokine Action

as follows: (1) inhibition of the ability of the cytokine to bind to its receptor (e.g., using soluble receptor or monoclonal antibody), (2) inhibition of the downstream events initiated by the cytokine binding to its receptor, and (3) inhibition of cytokine