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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
咨询甄准
- 保质期：
咨询甄准
- 英文名：
Tetramethylphosphonium bromide, 98%
- 库存：
1
- 供应商：
上海甄准
- CAS号：
4519-28-2
- 规格：
1g
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文献和实验James Hardwick CNBr Cleavage Procedure
tube containing 200 microliters of 100mg/ml CNBr in 70% formic acid. CNBr stocks are 200 milligrams/ml in 70% formic acid and are stored at -70°C. 70% formic acid is made from 98-100% formic acid and MQH2O. Optional: If you want to know
in cesium chloride-ethidium bromide gradients (1). Briefly, cells containing the desired plasmid or cosmid are harvested by centrifugation, incubated in a lysozyme buffer, and treated with alkaline detergent. Detergent solubilized proteins and membranes
Replication timing by density transfer
. To be certain, the gel should be blotted and probed for a known fragment. However, if you are familiar with the pattern expected for a particular restriction enzyme (e.g., EcoRI), you may be able to decide just from the ethidium bromide-stained gel
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