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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询甄准
- 保质期:
咨询甄准
- 英文名:
Cerium(III) trifluoromethanesulfonate, min. 98% (Cerium triflate)
- 库存:
1
- 供应商:
上海甄准
- CAS号:
76089-77-5
- 规格:
5g
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文献和实验量 锕(actinium) Ac 89 227.02 铝(aluminium) Al 13 26.98 镅(americium) Am 95
Native Chromatin Preparation and Illumina/Solexa Library Construction
the beads (10 min each wash): i. Twice with 1 mL of RIPA buffer ii. Twice with 1 mL of RIPA buffer + 0.3 M NaCl iii. Twice with 1 mL of LiCl buffer iv
Replication timing by density transfer
, then add Pronase (0.01-0.05 mg/ml final conc.). [You can add dry Pronase. I usually dissolve the Pronase in 5 ml of minimal medium, just to avoid having the dry Pronase stick to the sides of the flask.] 5. Hold at 37o C for 90-120 min
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