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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
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- 英文名:
Gt anti-Hu IgG(H&L) Polystyrene Particles
- 库存:
0.5% w/v, 14.0-17.9 um
- 供应商:
上海熹垣生物
- CAS号:
N/A
- 规格:
5 mL
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文献和实验do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10 the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody
Construction and Characterization of Adenovirus Vectors
. First-generation Ads have a large cloning capacity (5-14 kbp) and efficiently transduce a wide range of both quiescent and proliferating cell types. They are readily propagated to produce high-titer stocks (1011 -1013 vector particles from a 3-L culture
, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v
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