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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100⁺盒
- 英文名:
RNA Clean & Concentrator-5(DNase not Included)
- 保质期:
1年
- 供应商:
上海睿安生物13611631389
- 保存条件:
Room Temperature
- 规格:
50次/盒
Zymo Research货号R1015现货RNA纯化试剂盒-5上海睿安生物13611631389
Zymo Research货号R1016现货RNA纯化试剂盒-5上海睿安生物13611631389
Description
Performance
Technical Specifications
| Equipment | Microcentrifuge |
|---|---|
| Format | Spin-Column |
| Purity | RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8. |
| Sample Source | DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction |
| Size Range | Total RNA ≥ 17 nt |
| Yield | 10 µg RNA (binding capacity), ≥ 6 µl (elution volume) |


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文献和实验Zymo Research货号R1015现货RNA纯化试剂盒-5上海睿安生物13611631389
Zymo Research货号R1016现货RNA纯化试剂盒-5上海睿安生物13611631389
Description
Performance
Technical Specifications
| Equipment | Microcentrifuge |
|---|---|
| Format | Spin-Column |
| Purity | RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8. |
| Sample Source | DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction |
| Size Range | Total RNA ≥ 17 nt |
| Yield | 10 µg RNA (binding capacity), ≥ 6 µl (elution volume) |


Single tube confirmation PCR protocol
. For example, add 5 µl of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution from isolate #2 to PCR tubes 6-10 and so on. - Make a PCR master mix by combining: 638 µl water, 110 µl 10 x Taq Buffer, 11 µl NTP's, and 11 µl Taq
Single tube confirmation PCR protocol
tubes should contain 10 µl of the primer mix (5 µl of each primer). - Add the 5 µl of the Zymo treated cells to each of the 20 PCR reactions. For example, add 5 µl of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution
Single tube confirmation PCR protoco
of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution from isolate #2 to PCR tubes 6-10 and so on. - Make a PCR master mix by combining: 638 µl water, 110 µl 10 x Taq Buffer, 11 µl NTP's, and 11 µl Taq Polymerase. - Add 35
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