3,5-Difluoro-2-methoxyphenylbo

Silver Stain Protocol

from protein to protein. Caution: nucleic acids are stain. Fresh Solutions 0.02%  Sodium Thiosulfate (Na2S2O3) 0.1% AgNO3 for minigel and 0.15% for large gel. Stored in 4ºC before use. 2% Na2CO3 + 0.04% formaldehide. Prepare fresh. Note: Wear

【专题讨论】蛋白常用lysis buffer组成与作用详谈

是去垢的作用； PMSF PMSF即Phenylmethanesulfonyl fluoride，也称Phenylmethylsulfonyl fluoride或α-Toluenesulfonyl fluoride。中文名为苯甲基磺酰氟。分子式为C7H7FO2S，分子量为174.19。可抑制丝氨酸蛋白酶例如胰蛋白酶(trypsin)和胰凝乳蛋白酶(chymotrypsin)，也可抑制半胱氨酸蛋白酶和乙酰胆碱酯酶。有效浓度为0.1-1mM。难溶于水，且在水溶液中非常不稳

Incorporation of Phosphotyrosyl Mimetic 4- (Phosphonodifluoromethyl)phenylalanine (F2Pmp) Into Signal Transduction-Directed Peptides

-stable pTyr mimetics have been developed that retain certain of the chemical and pharmacological properties of pTyr itself. Among these, difluoro-phosphonomethyl phenylalanine (F2 Pmp) has shown widespread utility in a variety of signal transduction