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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询甄准
- 保质期:
咨询甄准
- 英文名:
[5-(Difluoromethyl)-3-methyl-1h-pyrazol-1-yl]acetic acid
- 库存:
1
- 供应商:
上海甄准
- CAS号:
512809-86-8
- 规格:
25mg
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文献和实验0.5 M hydrazine monohydrate (Aldrich) Pyridine Acetic acid
AFLP: not only for fingerprinting, but for positional cloning
acid from mini(micro)prep) 5 U EcoRI 5 U MseI 8.0 µL 5x-Pharmacia "One-Phor-All+" buffer [10x = 100 mM Tris-acetate, pH 7.5, 100 mM Mg-acetate, 500 mM K-acetate) w/ 250 ng/µg BSA (=5x OPA+- BSA) Q.S. to 40 µL w/ dH20 - incubate @ 37° C for ~ 3 hrs
AFLP: not only for fingerprinting, but for positional cloning
-HCl, pH 9.0 (@ 25° C), 1.0% Triton X-100; Mg-free) 1.2 µL 25 mM MgCl2 0.08 µL (0.4 U) Taq Pol (Promega) 14.42 µL dH2O PCR reactions (recipe is per rxn) 1 µL cut/ligated nucleic acid (from above) [equals 10 ng total nucleic acid)
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