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- 英文名：
EDA-GTPγS-ATTO-495
- 库存：
999
- 供应商：
上海维亦特生物
- CAS号：
N/A
浓度: 1.0 mM - 1.1 mM
分子式: C34H45N10O15P3S (free acid)
分子量: 958.77 g/mol (free acid)
性状: solution in water
存储: store at -20 °C
Shipping: shipped on dry ice
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文献和实验浓度: 1.0 mM - 1.1 mM
分子式: C34H45N10O15P3S (free acid)
分子量: 958.77 g/mol (free acid)
性状: solution in water
存储: store at -20 °C
Shipping: shipped on dry ice
GTPγS-induced Inhibition of Binding
GTPγS-induced Inhibition of Binding 1. Chemicals and Equipment DB Buffer, PB, cAMP, cAMP [2, 8- 3H] (25 Ci/mmol, 1.0 mCi/ml, 32, 000 nM), 10 mM GTPγS, Wacker silicone AR-20, Reagiergefab test tubes (Sarstedt), millipore
preparations, GTP can be substituted by 35 S-labeled guanosine 5′-O -(3-thio)triphosphate ([35 S]GTγS) and on agonist stimulation a stable [35 S]GTPγS-Gα complex will form and accumulate. Separation of 35 S-bound GTPγS-Gα complexes from free [35 S]GTPγS allows
[35S]GTPS Binding as an Index of Total G-Protein and G-Subtype-Specific Activation by GPCRs
preparations, GTP can be substituted by 35 S-labeled guanosine� 5′-O -(3-thio)triphosphate ([35 S]GTPγS) and on agonist stimulation a quasi-stable [35 S]GTPγS–Gα �complex forms and accumulates. Separation of [35 S]GTPγS–Gα complexes from free [35 S]GTPγS allows
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