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- 文献和实验
- 技术资料
- 保存条件：
咨询甄准
- 保质期：
咨询甄准
- 英文名：
S-[3-(N-(Ethyl-d5)maleimide)] Captopril
- 库存：
1
- 供应商：
上海甄准
- 规格：
1mg
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文献和实验Phycoerythrin conjugation protocol
(Molecular Probes) DTT Dithiothreitol NEM N-ethyl maleimide * 10 ml Sephadex G-10 columns (2) Separation column A long Sephacryl S-300 column
. Although GC method based on the preparation of N-heptafluorobutyryl isobutyl or ethyl esters (8 ) is selective and sensitive by flame photometric detection (FPD), this method is not applied to the analysis of biological samples. As the HPLC methods
Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells
, the reduced thiols are first blocked with N -ethyl-maleimide, then the oxidized thiols reduced with dithiothreitol and labelled with 5-iodoacetamidofluorescein. The method is illustrated by treating Jurkat T-lymphoma cells with hydrogen peroxide and monitoring
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