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- 2025年07月09日
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咨询甄准
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- 英文名：
rac-3’-O-Desethyl-3’-O-methyl Apremilast
- 库存：
1
- 供应商：
上海甄准
- 规格：
2.5mg
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文献和实验Optimizing Splice-Switching Oligomer Sequences Using 2-O-Methyl Phosphorothioate Chemistry
and then a set of oligomers complementary to the acceptor and donor splice sites, as well as intra-exonic regions predicted to contain exon splice enhancers, are designed and synthesized as 2′-O-methyl-modified bases on a phosphorothioate backbone (2OMeAOs
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
(dip 3-6 times or leave ~15-60 seconds). The section should not be too dark. The methyl green concentration can be varied if sections are generally too dark or too light. It's better to start with a lower concentration. dH2O 1-5 mins
filter sterililize; no DEPC treatment. Preparation of dNTP's. The quick protocol for ~100mM stock. dilute all four dNTP's (250mg of each) in 3.676ml of H2 O; + 424µl 5M NaOH; check the pH: ~0.5µl on pH-paper
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