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- 文献和实验
- 技术资料
- 保存条件:
咨询甄准
- 保质期:
咨询甄准
- 英文名:
8-Methylthiazolo[4',5':5,6]benzo[1,2-d]isothiazol-2-amine
- 库存:
1
- 供应商:
上海甄准
- CAS号:
13399-27-4
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文献和实验. Take the precipitate using a sterile transfer pipet (IWAKI 7801-002) (Do not suck! Wind the precipitate around the pipet.) or by centrifuging Dissolve the precipitate in (2/5 x X) ml TE containing 1 M NaCl and RNase A (1 to 10 ug/ml). Incubate at 55oC for 1 hr
.) or by centrifuging Dissolve the precipitate in (2/5 x X) ml TE containing 1 M NaCl and RNase A (1 to 10 ug/ml). Incubate at 55o C for 1 hr. Add equal volume of 2-propanol. Mix gently by swinging the tube slowly. Rinse
RLCS (Restriction Landmark cDNA Scanning) Protocol
of 11(W) x 350(L) x 3(D) mm. Remove any trace of the buffer. Pour 3 ml of 1x HB containing 1000 units of Hinf I and 0.01% BSA (enzyme solution). Seal the tray with Saran Wrap (incubate at 37o C for 2 hrs. Meanwhile, set up the 2-D electrophoresis
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