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文献和实验Techniques for generating human T-cell clones (TCCs) were first described nearly two decades ago (1 , 2 ). This was a direct consequence of the discovery of T-cell growth factor and the subsequent ability to propagate T-cells over extended
Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. We have performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. Materials 1X sterile PBS Anti
Generation of Human T Cell Clones
in several experimental settings. The availability of a population of human T lymphocytes deriving from the same progenitor with a unique phenotype and function (clone) may therefore be of help. This chapter describes the basic procedures that can be used
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