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文献和实验Immunoflourescence of Cultured Cells
antibodies Vectashield (Vector Labs) Protocol 1) Grow cells on L-lysine coated glass slips or cytospin cells onto charged glass slides. 2) Fix cells for 5 min. in neutral buffered formalin. 3) Permeabilize the nucleus by incubating
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
cytometric analysis, the OP9-DL1 will fluoresce in the FITC (FL1) channel. These cells can be gated-out using forward and side-scatter criteria, because these cells are larger and more granular than hematopoietic cells. Additionally, staining for CD
Multicolour 3D-FISH in vertebrate cells
, protocols for 3D-FISH on cultured mammalian cells (growing in suspension or adherently growing), and protocols for an efficient 3D-FISH on tissue sections, that have all been used successfully by our group. We restrict to protocols describing the labeling
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