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文献和实验Isolation of Untouched Human B Cells by Depletion of Non-B Cells
实验原理 Add a mixture of biotinylated monoclonal antibodies (Antibody Mix) against the non-B cells to the starting sample. Add Depletion MyOne SA Dynabeads® to bind the non-B cells during a short incubation. Separate the bead
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label a sample tube for each fluorochrome-conjugated rat or hamster antibody you are using, and add 1 drop of AbC™ RH capture beads
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