Anti-PIR-B-Biotin, mouse, REA8

用 CD3/CD28 做好 T 细胞激活与扩增

分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm

Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells

volume of Dynabeads taken  from the vial (step 2). 2.        Activation of Mouse T Cells 1)        Start with 8 × 104 purified T cells in 100-200 µl medium in a 96-well tissue culture plate. 2)        Add 2 µl Dynabeads Mouse T-Activator CD3/CD

is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea

that I routinely amplify; there are differences from gene to gene by my most optimal efficiencies run between 100 and 150 soluene, how much primer do you add? -aimikins- PRK- No, unfortunately the reference doesn't help with that. I did kind