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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
Need 1.5-2 x 107 cells from a 2 day culture. 1. Cells are harvested as normal, washed x 1 in PBS then taken up at conc. of 1.2 x 10 7 cells/ml in cold PBS. 2. Take x2 Biorad curvettes A. 0.9 ml (107 cells) B. 0.167 ml cells (2 x 106 cells
Depression‐Related Behavioral Tests
tests. J. Psychopharmacol. 23:797‐804. Ansorge, M. S., Zhoo, M., Liva, A., Hen, R., and Gingrich, J. A. 2004. Early life blockade of the 5‐HT transporter alters emotional
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