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文献和实验Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
v468.chapter 1 经典 Wnt/b-Catenin 信号通路 （英文完整版）
. (2005) Wnt and calcium signaling: beta-catenin-independentpathways. Cell. Calcium 38 , 439–446. 22. Zeng, X., Tamai, K., Doble, B., Li, S., Huang, H., Habas, R., et al. (2005) A dual-kinase mechanism for Wnt co-receptor phosphorylation
the Section 3, Methods; 48 h post-transfection, cell lysates were obtained, separated by SDS-PAGE, transferred to PVDFmembrane, and probed with antibodies to indicated proteins. Because β-catenin is highly expressed in Panc04.03 cells,changes in total level
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