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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询甄准
- 保质期:
咨询甄准
- 英文名:
Ethyl alpha-cyano-2-nitro-4-(trifluoromethyl)phenyl-acetate
- 库存:
1
- 供应商:
上海甄准
- CAS号:
13544-04-2
- 规格:
1g
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文献和实验tube mix: 15 µl dH2O (times number of samples + 1) 70 µl 1MTris Ph 8.0 (times number of samples + 1) 20 µl 4 mM Acetyl CoA (times number of samples + 1) and 5 µl 14 C CAM (times number of samples). 13. Add 0.5 ml Ethyl Acetate to each tube. 14
Ethyl Acetate to each tube. 14. Vortex 10" each tube & then mix by inverting for another 30". 15. Spin @ 14K RPM for 30"-1'. 16. Remove 450 µl of organic (upper) phase. 17. Speed vac for 15'. 18. Resuspend
is transferred to another tube and evaporated. The dried extract is reconstituted with ethyl acetate and 1 �L is injected onto the GCMS. Drugs are volatilized in the GC inlet and separated on a capillary column. In the EI source drugs become positively charged
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