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文献和实验, we may find much more references regarding this issue. http://books.google.com/books?id=3mgPzNFjcWAC&pg=PA51&lpg=PA51&dq=gsk+tyrosine+phosphorylation+and+activity&source=bl&ots=x5ueTldpez&sig=CqRFJleXtEBn9OfelhODPAc28xs&hl=en&ei=KerNSu-XGcem8Abm-qmABA&sa=X&oi=book_result&ct
Cell-Free Assay System for Ras- and Rap1-Dependent Activation of MAP-Kinase Cascade
the MEK activation (5 –7 ). Many MEK kinases have been identified: these include c-Raf-I (8 –10 ), B-Raf (11 –15 ), Mos (16 –17 ), and mStel 1 (11 –18 ). There are several lines of evidence that Ras is an upstream regulator of c-Raf
Two-hybrid analysis of genetic
and prey strains (see Protocol 1) One set of the following 150 x 15 mm plates for each test of interactions between an activation domain-tagged protein (in a prey strain)and 96 baits (bait strains): -u-h- Glu; -w Glu; YPD; -u-h-w Glu X-Gal; -u-h-w Gal/Raf
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