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文献和实验A Scintillation Proximity Assay for Fatty Acid Amide Hydrolase Compatible with Inhibitor Screening
that bind to human fatty acid amide hydrolase (FAAH). This SPA assay utilizes the specific binding of [3 H]-R (+)-methanandamide (3 H-MAEA), a competitive nonhydrolyzed FAAH inhibitor, to FAAH expressing microsomes and evaluates its displacement by FAAH
认为可能是继发于Ig的沉积。 3.天疱疮抗体与天疱疮的关系可由以下几方面来证明①DIF证明天疱疮病人皮损周围及“正常”皮肤处有IgG的沉积,且其荧光强度与病情的严重程度相平行;②血清中的Pab滴度与病情的严重程度相平行;③进行皮肤器官培养时,加入纯化的Pab可导致天疱疮样的棘层松解;④用血浆交换法去除Pab可导致天疱疮的缓解。 天疱疮抗体是针对表皮细胞膜多糖萼的抗体,其在体内与表皮细胞膜结合后可能使表皮细胞释放蛋白溶解酶如u �PA,u-PA在天疱疮发病
Analysis of Fatty Acid Amide Hydrolase Activity in Plants
acid by a membrane enzyme known as the fatty acid amide hydrolase (FAAH). Thus, FAAH represents an important target to better understand the function of these lipid mediators in numerous cellular processes. FAAH has been extensively characterized
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