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文献和实验Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris
The full-length and truncated forms of recombinant envelope (E) glycoprotein from Dengue virus type 1, Singapore strain S275/90 were expressed in the yeast, Pichia pastoris , using a secretory vector. A truncated form of the E protein
RT-PCR for the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virus
5A) genes showing (bold) HVR1 and 2, and ISDR, respectively. (C ) Location of PCR products (hatched areas) in relation to HVR and ISDR loci. The NS5A PCR precisely covers the NS5A gene. Diagram based on HCV-H-strain nucleotide and amino-acid sequence (5 ).
Use of Baculovirus Expression Vectors
), the prototype baculovirus strain and the virus most commonly used in expression vector systems, infects a number of lepidopteran species. Like most baculoviruses, it has two distinct morphological types. The budded virus form comprises single virus particles
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