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文献和实验Making antibodies to synthetic peptides
You'll need to couple the synthetic peptide hapten to a protein carrier, with a bifunctional reagent. For antibody development, we don't bother to use highly purified peptide. Crude (unpurified) peptide or partially purified peptide
Adrenal chromaffin granule (chromaffin vesicle) preparation
, Barbosa JA: Chromogranin A: localization and stoichiometry in large dense core catecholamine storage vesicles from sympathetic nerve. Brain Research 567:188-196, 1991. C. For our use of the prep for human pheochromocytoma granules, see: O
Making antibodies to synthetic pepti
You'll need to couple the synthetic peptide hapten to a protein carrier, with a bifunctional reagent. For antibody development, we don't bother to use highly purified peptide. Crude (unpurified) peptide or partially purified peptide
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