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文献和实验Methods for Manipulation of Transferrin-Binding Proteins
To obtain iron, a necessary nutrient, meningococci and several other human and veterinary pathogens have iron-acquisition systems, which are expressed in vivo during infection. One target of iron-acquisition systems is transferrin (Tf
Receptor-Mediated Transport of Drugs Across the BBB
receptor-mediated transcytosis or transport (RMT) across the BBB in vivo. Certain peptidomimetic monoclonal antibodies (mAb) for insulin receptor or transferrin receptor can also cross the BBB via RMT on the endogenous receptors. These mAb can act
ELISA-based assay for scatchard analysis of ligand-receptor interactions
, captured by a specific antibody coated on the surface of microwells, and quantitated with peroxidase-conjugated streptavidin in a colorimetric assay. Using this assay, Scatchard analysis was performed on the data for the specific binding of iron-loaded
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