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文献和实验Expression of VHH Antibody Fragments in Saccharomyces cerevisiae
proteins at high levels. Second, because S. cerevisiae does not secrete many homologous proteins during growth, an efficiently produced heterologous protein will be the major component of the growth medium. This, and the fact that S. cerevisiae
Mapping of Protein Transduction Pathways with Fluorescent Microscopy
, for instance the lysosomal organelles or the Golgi apparatus. Additionally, antibody staining of respective endocytic vesicles following the internalization of CPP–protein complexes will be discussed.
Synchronizing Protein Transport in the Secretory Pathway
, the protein is retained in the endoplasmic reticulum. At 10 min, it is located in the Golgi complex and after 60 min it is released in the extracellular medium and disappears from the intracellular space. (B ) At time 0 min, the protein is detected
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