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文献和实验of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins. A polyclonal antibody was raised against purified ST-IV. A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
v468.chapter 1 经典 Wnt/b-Catenin 信号通路 (英文完整版)
of the destruction complex, where it is thought toensure the efficient recruitment and anchoring of β-catenin. Theresident CK1 and GSK3 protein kinases sequentially phosphorylateconserved serine and threonine residues in the N-terminusof the trapped β-catenin (22
ELISPOT (Enzyme-linked ImmunoSPOT)
of cytokine-producing cells at the single cell level. The method employs the sandwich assay approach of the enzyme-linked immunosorbent assay (ELISA), with some variations. The ELISPOT capture antibody is coated aseptically onto a polyvinylidene difluoride
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