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文献和实验FEEDER PREPARATION BY GAMMA IRRADIATION
FEEDER PREPARATION BY GAMMA IRRADIATION All procedures should be carried out using sterile techniques at all times. Media for STOs/ SNL 76/7 cells is DMEM (high glucose, no pyruvate), 7% FBS and 1X GPS. 1. Gelatinize plates
James Hardwick angiotensin assay protocol
buffer (40 mM PIPES, pH 7.0, 10 mM MnCl2) at 4°C. 2. The kinase reaction is carried out at room temperature in a total volume of 20 microliters that contains: 15 microCi gamma-32P-ATP 5 microliters immunoprecipitate
Detection and Measurement of Radioactivity
resembling X-rays. An example of a gamma emitter is 131-I (iodine). Gamma radiation may accompany either alpha or beta particle emission. A traditional unit of radioactivity is the Curie (Ci), which is defined as that quantity of any radioisotope
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