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文献和实验. To each tube with the tail piece, add 0.5 ml of the following tail chop digestion buffer: 2.5 ml 1M Tris, pH 8 10.0 ml 0.5M EDTA 5.0 ml 10% SDS 31.5 ml ddI H2 O The final volume is 50 ml. To the amount needed
. for the duration of the harvesting process, after which we think these tails can be kept at 4℃ overnight without much trouble. To each tube with the tail piece, add 0.5 ml of the following tail chop digestion buffer: 2.5 ml 1M Tris, pH 8 10.0 ml 0.5M EDTA
Analysis of Chromatin Composition of Repetitive Sequences: The ChIP-Chop Assay
is not able to distinguish whether repeats that share identical sequences display a different composition of associated factors and, consequently, different functions. The ChIP-chop method provides a useful application to analyze the interaction of proteins
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