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文献和实验Purification and Characterization of Legionella U-Box-Type E3 Ubiquitin Ligase
Bacterial virulence proteins often mimic host eukaryotic proteins to modify or disturb host cellular �pathways. Increasing lines of evidence show that many bacterial effector proteins have E3 ubiquitin ligase activity. The effector protein
Reconstitution of CHIP E3 Ubiquitin Ligase Activity
CHIP, the carboxyl-terminus of Hsp70 interacting protein, is both an E3 ubiquitin ligase and an Hsp70 co-chaperone and is implicated in the degradation of cytosolic quality control and numerous disease substrates. CHIP has been shown
Studies of the Ubiquitin Proteasome System
1.1 ) Basic Protocol 12: Use of Dominant Negative Ubiquitin Proteins in Cells Materials Expression plasmids for mutant and wild‐type E2 or E3 Cultured cells to be transfected (e.g., U2OS or 293T) Nondenaturing
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