- ￥6238
- 赛默飞（Thermo Fisher）
- PA5114887
- 2025年07月15日
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文献和实验, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v
Construction and Characterization of Adenovirus Vectors
Reagents Agar Agarose (ultrapure, 1% w/v) Sterilize by autoclaving for 20 min at 121°C. Store at room temperature and melt in a microwave oven before use. Agarose gel (0.8%) and reagents for agarose gel electrophoresis Bacteria: BJ
by a quality control system, retrotranslocated into the cytosol and degraded. Soluble and membrane proteins were found to require a basic machinery for elimination. It is composed of (1) the E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3
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