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文献和实验BAND-SHIFT The procedure described in this chapter for the determination of affinity constants and kinetic dissociation constants by band-shift assay refers to an ideal antibody fragment (e.g., a scFv or an Fab fragment) binding
, and differentiation. More than 50 GSK-3 inhibitors representing a wide range of chemical structures have already been identified,and their utility in the treatment of type II diabetes mellitus, Alzheimer’s disease, bipolar diseases, cancer, andother human
. PBTB solution: 1x PBS, 0.1% Tween-20, 1% milk powder. 4. Detection of DIG-labeled probe: a. Biotinylated anti-DIG antibody followed by Streptavidin-HRP, recommended to obtain strongest signal: Biotin-conjugated mouse
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