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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
【求助】新手 请问western-blot测NF-KB、FLT3是用总蛋白提取试剂盒吗
xungufeng820 各位大侠: 小弟是新手,师兄们也没做过的,不知western-blot测NF-KB、FLT3是用总蛋白提取试剂盒吗?急盼回复!万分感谢! snowxyh 我用过总蛋白提取试剂盒提蛋白,之后western-blot检测NF-KB,但是感觉这样得到的蛋白浓度很稀,需要比较大的上样量才能检测出来,个人感觉,还是用传统的细胞匀浆裂解,离心提取上清,这样得到的蛋白浓度大,做试验结果好
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
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