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文献和实验of microinjecting cRNA to proteins of interest that have been coupled to a fluorescent protein using cyclin B1 as an example.
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
slurry (Fisher Scientific 45‐000‐116) Immunoprecipitation (IP) wash buffer (see recipe ) Phospho‐motif antibody
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
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