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文献和实验Strain Construction and Screening Methods for a Yeast Histone H3/H4 Mutant Library
A mutant library consisting of hundreds of designed point and deletion mutants in the genes encoding Saccharomyces cerevisiae histones H3 and H4 is described. Incorporation of this library into a suitably engineered yeast strain (e.g
Histone Acetylation and Deacetylation
transcription (1 ,2 ). DNA is packaged into chromatin, a highly organized and dynamic protein-DNA complex. The fundamental subunit of chromatin, the nucleosome, is composed of an octomer of four core histones, an H3/H4 tetramer and two H2A/H2B dimers, surrounded
, H4 。组蛋白的总重量和 DNA 的总重量大致相等,这个总量和比例在各种真核细胞中都是如此。
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